论文部分内容阅读
Objective: To study the effect of KATp channel opener and its possible mechanism on the sinoatrial node cells of neonatal rats which were cultured under simulated ischemia-reperfusion. Methods: Freshly isolated sinoatrial node (SAN) cells of neonatal rats were purified and cultured for 2 d, and then they were randomly divided into the control, simulated ischemia-reperfusion group (I/R group) , group intervened with KATp channel opener pinacidil (P + I/R group), KATP Channel blocking agent 5-HD (5-HD + I/R group) , and group with the 2 agents at same time (5-HD + P + I/R group) . The survival rate of cells was measured by flow cytometry and the content of intracellular calcium in the cells of each group was detected with laser confocal microscopy. Results: ① The survival rate of SAN cells in I/R group [ (51. 79±6. 28)% ] was remarkably significantly lower than in control [ (95. 08±10. 48)% ] (P < 0.001), and very significantly lower than in P + I/R group [ (63. 77±5. 35) % ] (P<0.01), however, those
Objective: To study the effect of KATp channel opener and its possible mechanism on the sinoatrial node cells of neonatal rats which were cultured under simulated ischemia-reperfusion. Methods: Freshly isolated sinoatrial node (SAN) cells of neonatal rats were purified and cultured for 2 d, and then they were randomly divided into the control, simulated ischemia-reperfusion group (I / R group), group intervened with KATp channel opener pinacidil (P + I / R group), KATP Channel blocking agent 5-HD HD + I / R group), and group with the 2 agents at same time (5-HD + P + I / R group). The survival rate of cells was measured by flow cytometry and the content of intracellular calcium in the cells of Results: ① The survival rate of SAN cells in I / R group [(51. 79 ± 6. 28)%] was remarkably significantly lower than in control [(95.08 ± 10. (P <0.001), and very significantly lower than in P + I / R group [(63.77 ± 5. 35)%] 1), however, those