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目的:研究miR-124在脑血流灌注不足大鼠中的动态表达变化,并探讨其在脑血流灌注不足中发挥的作用。方法:采用双侧颈总动脉结扎(Permanent bilateral comman carotid artery occlusion或Two-vessel occlusion,2VO)制备大鼠脑缺血模型,用qRT-PCR的方法在不同的时间点(术后1、7、14、21、28 d)检测miR-124的表达变化。并在SH-SY5Y细胞系中采用流式细胞仪研究低氧时过表达miR-124对细胞周期的影响。结果:与对照组比较,2VO术后大鼠脑皮质血流经历了先下降再上升的动态变化,而miR-124也呈现先下降再上升的动态过程,于术后第7天达到最低(P=0.036);在低氧环境中,过表达miR-124可以将神经细胞系SH-SY5Y的阻止在G1期。结论:miR-124可能参与了大鼠脑血流灌注不足后脑缺血的发生发展,为治疗脑缺血提供了一种新的治疗策略。
Objective: To investigate the dynamic expression of miR-124 in cerebral hypoperfusion in rats and to explore its role in cerebral hypoperfusion. Methods: The rat model of cerebral ischemia was induced by bilateral bilateral common carotid artery occlusion (2VO). The rats were sacrificed at different time points (1, 7, 14, 21, 28 d) to detect the expression of miR-124. The effects of miR-124 overexpression on cell cycle in hypoxia were investigated by flow cytometry in SH-SY5Y cell line. Results: Compared with the control group, the cerebral cortex blood flow of rats with 2VO experienced first and then decreased dynamic changes, and miR-124 also showed the dynamic process of first decreasing and then increasing, reaching the lowest = 0.036). In hypoxic environment, overexpression of miR-124 blocked the neuronal cell line SH-SY5Y in G1 phase. Conclusion: miR-124 may be involved in the development of cerebral ischemia after cerebral hypoperfusion in rats, which provides a new therapeutic strategy for the treatment of cerebral ischemia.