论文部分内容阅读
目的观察不同频率超声联合靶向微泡、尿激酶在体溶栓后对微循环功能的影响。方法 18只新西兰大白兔单侧股动脉制成富含血小板的混合性血栓模型,分为A、B、C3组各6只。均通过靶向微泡携带尿激酶在超声照射30 min下辅助溶栓,其中A组超声频率1.6 k Hz,B组2.2 k Hz,C组2.8 k Hz。应用脉冲多普勒血流仪持续监测血流流速,对血流量变化特点进行分析,并对各组胫前肌行HE染色,比较不同频率超声的溶栓作用及对微循环功能变化的影响。结果溶栓后120 min时,三组血流量变化比较,差异有统计学意义(P<0.05),其中B组与A、C组比较实现了完全再通。A、C组均有未溶通或者微栓塞情况发生。经HE染色后发现C组微循环末端有微小栓子,成分为由嗜酸性同质性纤维素构成,镜下呈粉红色。结论超声频率2.2 MHz的条件下溶栓可以实现血栓的完全溶解,远端未见微栓子形成,栓子处于完全溶解状态。
Objective To observe the effects of different frequencies of ultrasound combined with targeted microbubbles and urokinase on the microcirculation function after thrombolysis in vivo. Methods Twenty - eight New Zealand white rabbits were made into a platelet - rich mixed thrombus model by unilateral femoral artery, and divided into A, B and C3 groups. Thrombolytic therapy was assisted by targeted microvesicle embolization with urokinase for 30 min after ultrasound irradiation. The group A had an ultrasonic frequency of 1.6 k Hz, a group B of 2.2 k Hz and a group C of 2.8 k Hz. The pulsatile Doppler flowmeter was used to monitor the blood flow velocity continuously. The characteristics of blood flow were analyzed. HE staining was performed on the anterior tibial muscle of each group to compare the thrombolytic effect of different frequency ultrasound and the changes of microcirculation function. Results At 120 min after thrombolysis, the changes of blood flow in the three groups were statistically significant (P <0.05). Complete recanalization was achieved between group B and groups A and C. A, C group have not dissolved or micro-embolism occurred. After HE staining found microcirculation of C group micro-emboli, composed of eosinophilic cellulose composition, microscopic pink. Conclusion Thrombolysis can be achieved by thrombolysis under the condition of 2.2 MHz ultrasonic frequency. There is no micro-embolus formation in the distal end and the embolus is completely dissolved.