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目的：探讨多药耐药相关蛋白（MRP）基因表达与原发上皮性卵巢癌（PEOC）耐药的关系及其临床意义。方法：应用半定量RT-PCR技术，检测37份PEOC，10份正常卵巢上皮组织和20份正常女性外周血单个核细胞（PBMC）新鲜标本MRP基因的表达，用MTT法检测PEOC患者的原代癌细胞对临床常用抗癌药物的敏感性。结果：（1）PEOCMRP基因表达水平明显高于正常卵巢上皮组织（P＜0．05），与PBMC差异无显著性（P＞0．05）；（2）MRP基因表达与PEOC临床期别及组织学类型无关，但随组织学级别升高而升高（P＜0．05）；（3）MRP基因高表达组MMC、ADM、E-ADM、VCR、VP16和CDDP对PEOC细胞抑制率与低表达组差异有显著性，余则无显著性；（4）MRP和MDR1基因表达里正相关（r＝0．213，P＜0．01），且与无进展生存期有关，而与总体生存期无关。结论：MRP基因表达与PEOC耐药有关，是抗MDR相关药物的重要原因之一。MRP基因表达的检测对临床用药有指导意义，对临床疗效判断和患者预后预测有一定价值。
Objective: To investigate the relationship between multidrug resistance-related protein (MRP) gene expression and drug resistance in primary epithelial ovarian cancer (PEOC) and its clinical significance. Methods: The expression of MRP gene in fresh specimens of 37 samples of PEOC, 10 normal ovarian epithelial tissues and 20 normal female peripheral blood mononuclear cells (PBMCs) were detected by semi-quantitative RT-PCR. The primary Sensitivity of cancer cells to commonly used anticancer drugs. Results: (1) The expression of PEOCMRP gene was significantly higher than that of normal ovarian epithelial tissue (P <0.05), but not significantly different from that of PBMC (P> 0.05). (2) (P <0.05). (3) The inhibitory rates of MMC, ADM, E-ADM, VCR, VP16 and CDDP with high MRP gene expression in PEOC cells were significantly correlated with the histological grade (4) There was a positive correlation between MRP and MDR1 gene expression (r = 0.213, P <0.01), and with progression-free survival, but not with overall Survival has nothing to do. Conclusion: MRP gene expression is associated with PEOC resistance and is one of the important reasons for MDR related drugs. The detection of MRP gene expression is of guiding significance to clinical medication, and has certain value for judging clinical efficacy and prognosis of patients.