论文部分内容阅读
目的:制备骨形成蛋白2/珍珠层粉/壳聚糖复合多孔支架,观察支架生物性能。方法:采用冷冻干燥法制备骨形成蛋白2/珍珠层粉/壳聚糖多孔支架。用光学显微镜和扫描电子显微镜观察支架表面形貌及孔径大小,用比重瓶法检测支架孔隙率,热重分析探讨支架的热稳定性,用微力试验机进行压缩性能测试,并将支架与兔骨髓间充质干细胞共培养检测细胞黏附性能,将支架埋置大鼠皮下观察其炎症反应。结果与结论:制备的骨形成蛋白2/珍珠层粉/壳聚糖支架孔径大小为100~300μm,孔隙率为91.64%,压缩应力达3.37MPa,与细胞共培养贴附较好,有良好的组织相容性,提示该支架可做为组织工程支架材料应用于临床上骨组织缺损的修复。
OBJECTIVE: To prepare bone morphogenetic protein 2 / nacre powder / chitosan composite porous scaffold and observe its biological performance. Methods: Bone morphogenetic protein 2 / nacreous powder / chitosan porous scaffolds were prepared by freeze-drying. The surface morphology and pore size of scaffolds were observed by light microscopy and scanning electron microscopy. The porosity of scaffolds was measured by pycnometer method. The thermal stability of scaffolds was investigated by thermogravimetric analysis. The compressive properties of scaffolds were tested by micromechanical testing machine. Mesenchymal stem cells co-cultured to detect cell adhesion, the scaffold buried rat subcutaneous observation of its inflammatory response. RESULTS AND CONCLUSION: The pore size of the prepared bone morphogenetic protein 2 / nacre powder / chitosan scaffolds was 100-300 μm with a porosity of 91.64% and a compressive stress of 3.37 MPa. The scaffolds co-cultured well with the cells and had good Tissue compatibility, suggesting that the scaffold can be used as a tissue engineering scaffold material for the clinical repair of bone defects.