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目的探讨解偶联蛋白2(UCP2)基因-866 G/A、A55V和3′非翻译区(UTR)插入/缺失(I/D)多态性与维吾尔族自然长寿的关联。方法以新疆和田地区191名维吾尔族自然长寿个体为研究对象,以53名75岁前自然死亡的同地区、同民族个体作为对照。采用PCR-限制长度片段多态性(PCR-RFLP)和PCR-直接测序法对UCP2基因-866 G/A、A55V和3′-UTR I/D多态性进行分型。结果启动子-866G/A变异AA基因型与长寿相关,A55V多态性和3′-UTR I/D多态性基因型、等位基因分布在长寿组和对照组之间无差异。结论 UCP2基因启动子-866G/A多态性与长寿相关。
Objective To investigate the association of UCP2 gene-866 G / A, A55V and 3 ’untranslated region (UTR) insertions / deletions (I / D) polymorphisms with the natural longevity of Uygur nationality. Methods 191 natural and longevity individuals from Uyghur in Hetian were used as subjects. Fifty-three individuals living in the same area before the age of 75 were used as controls. UCP2 gene -866 G / A, A55V and 3’-UTR I / D polymorphisms were genotyped by PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-direct sequencing. Results The AA genotype of promoter -866G / A was associated with longevity. The A55V polymorphism and 3’-UTR I / D polymorphism genotype and allele distribution were not different between longevity group and control group. Conclusion UCP2 gene promoter -866G / A polymorphism is associated with longevity.