应用异型双功能基化合物N-羟琥珀酰亚胺碘乙酸酯(NHSIA)与蓖麻毒素(Ricin)反应,产生碘乙酰毒素,再与暴露巯基的单克隆抗体(McAb)-PDP反应,通过Sephadex G-200及Sepharose-4B分离,得到缺乏半乳糖结合点免疫毒素。该结合物对人肝癌细胞株Bel7404具有良好的导向杀伤作用,在10~(-9)mol/L浓度时杀伤率为44.1％,非常显著地高于对非靶细胞HeLa的杀伤力(P<0.01)。在10~(-7)mol/L浓度时,对HeLa的杀伤率为39.7％,0.1mol/L半乳糖能够降低这一杀伤作用。 N-hydroxysuccinimidyl iodoacetate (NHSIA), a heterobifunctional compound, was used to react with ricin to produce iodoacetyl toxin, which was then reacted with a monoclonal antibody (McAb) -PDP that exposed to sulfhydryl Sephadex G-200 and Sepharose-4B were isolated, resulting in lack of galactose binding spots immunotoxins. The conjugate has good targeting and killing effect on human hepatocellular carcinoma cell line Bel7404, and the killing rate is 44.1% at the concentration of 10-9 mol / L, which is significantly higher than that of the non-target cell HeLa (P < 0.01). At a concentration of 10 ~ (-7) mol / L, the killing rate of HeLa was 39.7%, and 0.1 mol / L galactose could reduce this killing effect.