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为有效缓解盐胁迫对大豆种子萌发的抑制,采用hemin与CaCl2、水杨酸(SA)、脯氨酸(Pro)和KNO3等物质的组合,对苏豆5号大豆种子的发芽率、发芽指数以及根与下胚轴的长度进行测定分析。结果表明:在盐胁迫(150 mmol·L-1NaCl)条件下,大豆种子的发芽率、发芽指数等指标均有明显下降,而一定浓度的CaCl2、SA、Pro、KNO3和hemin均能缓解萌发抑制;CaCl2、Pro与hemin联用处理对缓解大豆种子盐胁迫下萌发抑制比其单独处理具有更好的效果,可有效提高大豆种子在盐胁迫条件下的平均根长、发芽率和发芽指数。进一步研究发现,联用处理还有助于提高大豆种子萌发过程中的淀粉酶和过氧化物酶活性,其中120μmol·L-1hemin+1.5 mmol·L-1CaCl2与80μmol·L-1hemin+10mmol·L-1Pro为最佳联用浓度。
In order to effectively alleviate the inhibition of soybean seed germination under salt stress, the combination of hemin with CaCl2, salicylic acid (SA), proline (Pro) and KNO3 were used to study the germination rate, germination index And the length of root and hypocotyls were measured and analyzed. The results showed that under the salt stress (150 mmol·L-1NaCl), the germination rate and germination index of soybean seeds decreased obviously, while the concentrations of CaCl2, SA, Pro, KNO3 and hemin all alleviated the germination inhibition The combination of CaCl2, Pro and hemin could relieve the germination of soybean seeds under salt stress more effectively than the single treatment, which could effectively increase the average root length, germination rate and germination index of soybean seeds under salt stress. Further study found that combined treatment also helps to improve the amylase and peroxidase activity of soybean seed germination process, in which 120μmol·L-1hemin + 1.5mmol·L-1CaCl2 and 80μmol·L-1hemin + 10mmol·L -1Pro for the best combined concentration.