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目的调查研究一起羊养殖场中发生的人畜共患羊种布鲁氏菌病(布病)暴发疫情的流行病学和分子特征。方法血清学实验采用试管凝集试验检测布病抗体,采用纸片法检测菌株的药物敏感性,采用多位点可变数目串联重复序列分析(MLVA)和多位点序列分析(MLST)分析菌株的分子特征。结果工作人员布鲁氏菌抗体阳性率为7/18,饲养羊的阳性率为5/8。2名工作人员血液分离培养出羊种布鲁氏菌(菌株编号:CQ-18和CQ-11),3只羊的血液分离培养出羊种布鲁氏菌(菌株编号:CQ-154、CQ-159和CQ-237)。药敏实验结果:5株菌对环丙沙星、左氧氟沙星、强力霉素、头孢曲松、诺氟沙星、米诺霉素、莫西沙星、氧氟沙星、利福平和链霉素敏感,CQ-154和CQ-237菌株对复方新诺明敏感,CQ-159、CQ-18和CQ-11菌株对复方新诺明耐药,5株菌均对阿奇霉素和克拉霉素耐药。MLVA分析发现,CQ-154菌株在BRU16位点少了一个串联重复序列,菌株CQ-18在BRU04位点增加了一个串联重复序列,MLST分析5株菌株ST型为8型。结论布鲁氏菌长期在动物体内存在,是人畜感染布病无法根治的一个关键问题。在我国首次发现对复方新诺明耐药的羊种布鲁氏菌菌株。
Objective To investigate the epidemiological and molecular characteristics of an outbreak of brucellosis (brucellosis) in zoonoses in sheep farms. Methods Serological tests were used to detect the antibody of brucellosis by tube agglutination test. The drug sensitivity of the strains was tested by disk test. MLVA and MLST were used to analyze the susceptibility of the strains. Molecular characteristics. Results The positive rate of brucellosis was 7/18, the positive rate of feeding sheep was 5 / 8.2. The blood of the workers was isolated from Brucella abortus (strain number: CQ-18 and CQ-11 ), Brucella bovine (strain no. CQ-154, CQ-159 and CQ-237) were isolated from the blood of 3 sheep. Susceptibility test results: Five strains were sensitive to ciprofloxacin, levofloxacin, doxycycline, ceftriaxone, norfloxacin, minocycline, moxifloxacin, ofloxacin, rifampicin and streptomycin , CQ-154 and CQ-237 strains were sensitive to cotrimoxazole. CQ-159, CQ-18 and CQ-11 strains were resistant to cotrimoxazole. All five strains were resistant to azithromycin and clarithromycin. MLVA analysis showed that CQ-154 had a less tandem repeat at BRU16, CQ-18 added a tandem repeat at BRU04, and MLST showed that ST strain was type 8 by MLST. Conclusion Brucella is present in animals for a long time and is a key issue that can not be cured by brucellosis. In China for the first time found to compound cotrimoxazole resistant strains of brucella.