论文部分内容阅读
Objective To prepare liver-targeted baicalin solid lipid nanoparticles(BSLNs) and to study their in vitro anti-oxidative activity.Methods BSLNs were prepared by emulsification ultrasonic dispersion method and characterized by transmission electron microscopy and laser particle size distribution;The tissue in vivo distribution was detected by pharmacokinetics;In vitro anti-superoxide dismutase(SOD) activity and reduction capacity of BSLNs were determined;The ability of removing hydroxyl radical was determined by phenanthroline-Fe2+ oxidation.Results The best prescription was baicalin-soybean lecithin-glyceryl monostearate-poloxamer 188(1:5:15:30);The encapsulation efficiency and drug loading were 84.7% and 5.65%,respectively,mean size of particles was(68.6 ± 8) nm,Zeta potential was 22.13 mV;The in vitro anti-oxidant results showed that BSLNs had a significant inhibitory effect on SOD and a strong reducing capacity as well as a removing hydroxide radical ability.The targeting rate of BSLNs was 6.931 for liver.Conclusion The results demonstrate that BSLNs could enhance the liver targeting ability and in vitro anti-oxidative activity significantly.
Objective To prepare liver-targeted baicalin solid lipid nanoparticles (BSLNs) and to study their in vitro anti-oxidative activity. Methods BSLNs were prepared by emulsification ultrasonic dispersion method and characterized by transmission electron microscopy and laser particle size distribution; The tissue in vivo distribution was detected by pharmacokinetics; In vitro anti-superoxide dismutase (SOD) activity and reduction capacity of BSLNs were determined; The ability of removing hydroxyl radical was determined by phenanthroline-Fe2 + oxidation. Results of the best prescription was baicalin-soybean lecithin- glyceryl monostearate- The encapsulation efficiency and drug loading were 84.7% and 5.65%, respectively, mean size of particles was (68.6 ± 8) nm, Zeta potential was 22.13 mV; The in vitro anti- oxidant results showed that BSLNs had a significant inhibitory effect on SOD and a strong reducing capacity as well as a hydroxide removal ability. The targeting rate of B SLNs was 6.931 for liver. Confluence The results demonstrate that BSLNs could enhance the liver targeting ability and in vitro anti-oxidative activity significantly.