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为了探讨绿蒜75%乙醇提取物的正己烷萃取物(简称绿蒜正己烷萃取物:GGHE)对H22荷瘤小鼠的抑瘤作用及对其免疫功能的影响,建立H22荷瘤小鼠模型。将60只小鼠平均分为6组:空白对照组、模型组、阳性对照组(环磷酰胺CTX,20 mg/kg)、GGHE低中高剂量组(L-GGHE:75 mg/kg;M-GGHE:150 mg/kg;H-GGHE:300 mg/kg),连续给药14 d。测定小鼠体重、水食量、肝脏中SOD、GSH-Px、CAT、ALT、AST的活性和MDA的含量;测定小鼠血清中细胞因子TNF-α、IL-2和VEGF的含量;计算水食效率、瘤体积、抑瘤率、脏器指数;分析肿瘤组织的病理特征。实验结果表明:M-GGHE的抑瘤率达71.48%。与模型组相比,GGHE能显著提高小鼠水食效率、胸腺及脾脏指数以及IL-2,TNF-α的水平,降低VEGF的表达。不同程度的提高肝脏组织中抗氧化酶SOD、GSH-Px和CAT的活力、降低MDA的含量以及转氨酶ALT和AST的活力。结论:GGHE对H22荷瘤小鼠的肿瘤生长有一定的抑制作用,改善了荷瘤小鼠的生存质量,提高了荷瘤小鼠的抗氧化水平和免疫功能。本实验提示可将绿蒜75%乙醇提取物的正己烷萃取物开发成治疗肝癌的候选药物。
In order to investigate the anti-tumor effect of n-hexane extract of Garlic 75% ethanol extract (GGHE) on H22 tumor-bearing mice and its immune function, a H22 tumor-bearing mouse model was established . Sixty mice were equally divided into six groups: control group, model group, positive control group (cyclophosphamide CTX 20 mg / kg), GGHE low medium and high dose group (L-GGHE: 75 mg / GGHE: 150 mg / kg; H-GGHE: 300 mg / kg) for 14 days. The activities of SOD, GSH-Px, CAT, ALT, AST and the contents of MDA in the liver were measured. The contents of cytokines TNF-α, IL-2 and VEGF in the mice were measured. Efficiency, tumor volume, tumor inhibition rate, organ index; analysis of tumor tissue pathological features. The experimental results showed that the inhibition rate of M-GGHE reached 71.48%. Compared with the model group, GGHE can significantly improve the water food efficiency, thymus and spleen index, IL-2, TNF-α levels and reduce the expression of VEGF in mice. To some extent, the activities of antioxidant enzymes SOD, GSH-Px and CAT in liver tissue were increased, the content of MDA and the activities of ALT and AST were increased. Conclusion: GGHE can inhibit the tumor growth of H22 tumor-bearing mice, improve the quality of life of tumor-bearing mice, and enhance the anti-oxidation and immune function of tumor-bearing mice. This experiment suggests that the n-hexane extract of 75% ethanol extract of green garlic can be developed into a candidate drug for the treatment of liver cancer.