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目的:研究灯盏花制剂对缺血性脑损伤脑成像的影响及脑组织代谢物的作用。方法:雄性SD大鼠23只,随机分成缺血损伤组(n=11)和灯盏花治疗组(n=12),分别用于核磁共振和大脑表面血管的观察。颈部正中切口,线栓法造成右大脑中动脉血供阻断(MCAO)。缺血2h后,立即腹腔注射灯盏花注射液(1.5mg·kg-1体重)。采用核磁共振T2加权成像(T2WI)和局域质子谱(1H-MRS)技术,研究大脑成像和神经元代谢物N-乙酰天门冬氨酸(NAA)、肌酸/磷酸肌酸(Cr/PCr)、胆碱(Cho)和乳酸(Lac)的变化。结果:灯盏花治疗组的高信号强度区信号较同时刻同时间点缺血损伤组减弱、体积小,T2值减小,NAA值大,Cr/NAA和Cho/NAA比值小,未见乳酸峰;大脑表面的血管呈扩张状态。结论:灯盏花有助于缺血脑组织的血液循环重建,改善神经元的代谢。
Objective: To study the effect of Erigeron breviscapus preparation on brain imaging of ischemic brain injury and the effect of brain metabolites. METHODS: Twenty-three male Sprague-Dawley rats were randomly divided into ischemic injury group (n=11) and breviscapus treatment group (n=12), which were used for MRI and observation of blood vessels on the brain surface, respectively. In the midline neck incision, the occlusion of the right middle cerebral artery (MCAO) was caused by a suture method. After 2 hours of ischemia, breviscapine injection (1.5 mg·kg-1 body weight) was intraperitoneally injected. Using nuclear magnetic resonance T2 weighted imaging (T2WI) and local proton spectroscopy (1H-MRS) techniques to study brain imaging and neuronal metabolites N-acetylaspartate (NAA), creatine/phosphocreatine (Cr/PCr Change of Choline (Cho) and Lactic Acid (Lac). RESULTS: The high signal intensity signal of Erigeron breviscapus treatment group was weaker and smaller than that of the ischemic injury group at the same time point. The T2 value was decreased, the NAA value was large, and the ratio of Cr/NAA and Cho/NAA was small. No lactate peak was observed. The blood vessels on the surface of the brain are in an expanded state. Conclusion: Erigeron breviscapus helps rebuild the blood circulation of ischemic brain tissue and improves the metabolism of neurons.