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利用抗和感黑胫病的白肋烟品种Burley37(P1)、Burley67(P2)配制F1代经花药培养获得的DH群体,进行了黑胫病抗性的遗传分析。将P1、P2和DH群体连续两年种植并人工接种黑胫病(Phytophthora parasiticavar.nicotianae)0号生理小种,同时调查发病率和计算病情指数,并采用植物数量性状主基因+多基因混合遗传模型分析法对黑胫病抗性进行遗传分析,估算DH群体的其他遗传参数。结果表明:黑胫病发病率和病情指数分别由2对具有互补作用的主基因+多基因(E-1-7)和2对具有等加性作用的主基因+多基因(E-1-3)控制;主基因遗传率分别为54.14%和50.68%;多基因遗传率分别为26.60%和19.31%;多基因有效因子数分别为0.61个和1.31个。因此,白肋烟黑胫病抗性主要由2对主基因及多基因控制。在育种实践中,烟草品种对黑胫病抗性的遗传相对比较稳定,但温度和湿度等环境因素在一定程度上也影响该病的发生与流行。
The DH population obtained from the anther culture of Burley37 (P1) and Burley67 (P2) with anti-susceptible black shank and F1 generation was used for genetic analysis of black shank resistance. The P1, P2 and DH populations were planted for two consecutive years and inoculated with the physiological race 0 of Phytophthora parasiticavar.nicotianae. The morbidity and the disease index were also investigated. The dominant gene + polygene mixed inheritance Model analysis was used to analyze the resistance of black shank and to estimate other genetic parameters of the DH population. The results showed that the incidence and severity index of black shank were composed of two pairs of major genes + polygene (E-1-7) and two pairs of major genes plus polygene (E-1- 3) control. The heritabilities of the major genes were 54.14% and 50.68% respectively. The rates of polygene inheritance were 26.60% and 19.31%, respectively. The effective number of multiple genes were 0.61 and 1.31, respectively. Therefore, the susceptibility of Burley black shank is dominated by two major genes and multiple genes. In the breeding practice, the inheritance of tobacco varieties to black shank resistance is relatively stable, but environmental factors such as temperature and humidity also affect the occurrence and prevalence of the disease.