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小麦白粉病(Blumeria graminis f.sp.tritici)是严重影响小麦生产的重要病害之一,培育和应用抗病品种是有效控制和减少病害的最经济有效的方法。野生二粒小麦是硬粒小麦和普通小麦的四倍体野生祖先种,是小麦抗病性遗传改良的重要基因资源。本研究利用来自以色列的野生二粒小麦WE29与普通小麦杂交,再用普通小麦连续回交和自交,育成高抗白粉病小麦新品系3D258(系谱为燕大1817/WE29//5*87-1,BC4F6)。将3D258和高感小麦白粉病的普通小麦品种薛早配制杂交组合,对其F1、F2代分离群体和F3代家系进行白粉病抗性鉴定和遗传分析。结果表明3D258携带抗白粉病显性单基因,暂命名为MlWE29。利用集群分离分析法(BSA)和分子标记分析,发现6个SSR标记(Xgwm335、Xgwm213、Xgwm639、Xwmc415、Xwmc289和Xwmc75)和5个EST-STS标记(BE494426、BE442763、CD452476、BE445282和BE407068)与抗白粉病基因MlWE29连锁。利用中国春缺体-四体系、双端体系和缺失系将抗白粉病基因MlWE29及其连锁标记物理定位于5BL染色体的0.59–0.79区域。这一普通小麦抗白粉病种质资源的创制及其连锁分子标记的建立为小麦抗病基因分子标记辅助选择、基因积聚和分子育种提供了新的物质基础。
Blumeria graminis f.sp. tritici is one of the most important diseases that seriously affect wheat production. Cultivating and applying disease-resistant cultivars is the most cost-effective way to effectively control and reduce the disease. Wild bred wheat is a tetraploid wild ancestor of durum wheat and common wheat and is an important genetic resource for genetic improvement of wheat disease resistance. In this study, WE29 was crossed with common wheat with wild wheat WE29 from Israel, and then backcrossed and selfed with common wheat. The new wheat line 3D258 with high resistance to powdery mildew was bred (genealogy YanDa 1817 / WE29 // 5 * 87- 1, BC4F6). The hybrid combinations of 3D258 and common wheat cultivars with high susceptibility to wheat powdery mildew were crossed with Xuexue to identify and genetic analyze the resistance of powdery mildew to F1, F2 segregated populations and F3 generation. The results showed that 3D258 carry powdery mildew dominant single gene, tentatively named MlWE29. Six SSR markers (Xgwm335, Xgwm213, Xgwm639, Xwmc415, Xwmc289 and Xwmc75) and five EST-STS markers (BE494426, BE442763, CD452476, BE445282 and BE407068) were found to be compatible with molecular marker analysis using cluster analysis (BSA) Powdery Mildew Resistance Gene MlWE29 Chain. The MlWE29 and its linkage markers of powdery mildew resistance gene were physically located in the 0.59-0.79 region of chromosome 5BL by using the Chinese Spring Genome-Quadruplex, Double-Terminal and Deletion Lines. The establishment of common wheat powdery mildew resistance germplasm resources and establishment of linkage molecular markers provide a new material basis for molecular marker-assisted selection, gene accumulation and molecular breeding of wheat disease resistance genes.