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目的研究中国特有红树植物海南海桑内生真菌Bionectriaochroleuca HHS111023的次生代谢产物及其生物活性。方法利用多种柱色谱技术对次生代谢产物进行分离纯化;通过核磁与质谱数据分析结合理化常数及文献比对,鉴定化合物的结构;分别采用纸片琼脂扩散法和MTT法对化合物的抗菌活性和细胞毒活性进行测试。结果从海南海桑内生真菌Bionectria ochroleuca HHS111023的次生代谢产物中分离鉴定了7个化合物,其中4个Lasiodiplodin型大环内酯,1个链状脂类化合物和2个苯的衍生物,分别是:Lasiodiplodin(1)、(R)-de-O-methyllasiodiplodin(2)、(5S)-5-hydroxylasiodiplodin(3)、(5R)-5-hydroxylasiodiplodin(4)、methyl(E)-11,12,15-trihydroxyoctadec-13-enoate(5)、对羟基苯乙醇(6)、对羟基苯乙酸甲酯(7)。化合物1和2分别对白色念珠菌和青枯雷尔氏菌具有抗菌活性,且分别对SGC-7901和K-562具有体外细胞毒活性。结论化合物1~4为首次从淡色生赤壳菌Bionectria ochroleuca次生代谢产物中分离得到,化合物5为首次分离自微生物次生代谢产物;海南海桑内生真菌Bionectria ochroleuca HHS111023能产生具有抗菌和细胞毒活性的化合物。
Objective To study the secondary metabolites and their bioactivities of endophytic fungus Bionectria chroleuca HHS111023, a endemic mangrove plant from Hainan, China. Methods The secondary metabolites were isolated and purified by a variety of column chromatography techniques. The structures of the compounds were identified by NMR-MS and physico-chemical constants and literature comparison. The antibacterial activities of the compounds were determined by agar diffusion method and MTT method respectively. And cytotoxic activity was tested. Results Seven compounds were isolated from the secondary metabolites of endophytic fungus Bionectria ochroleuca HHS111023, of which four Lasiodiplodin macrolides, one chain lipid compound and two benzene derivatives, respectively Are: Lasiodiplodin (1), (R) -de-O-methyllasiodiplodin (2), (5S) -5-hydroxylasiodiplodin , 15-trihydroxyoctadec-13-enoate (5), p-hydroxyphenethyl alcohol (6), and p-hydroxyphenylacetate (7). Compounds 1 and 2 have antibacterial activity against C. albicans and R. solanacearum, respectively, and have in vitro cytotoxic activity against SGC-7901 and K-562, respectively. Conclusions Compounds 1-4 were isolated from the secondary metabolites of Bionectria ochroleuca for the first time. Compound 5 was isolated from the secondary metabolites of microorganisms for the first time. Endophytic fungi Bionectria ochroleuca HHS111023 from Hainan seaweed could be produced with antibacterial and cellular Toxic active compounds.