用塑料袋制菌种,常用棉塞或扎线封袋口,前者在灭菌时棉塞易受潮而增加污染率,后者则由于通气差而导致后期菌丝长速慢、纤细长势不良。我们采用厦门市厦青塑料制品厂生产的无棉盖体塑料双套环制菌种,取得了良好的效果。 1 无棉盖体结构 分套环和盖两部分。盖中央有一直径1.1cm的孔,下垫一层无棉盖体,盖体下衬一张有6个小孔塑料薄片。使用时在套环上盖上盖子,压紧即可。 2 试验方法 基质配方为木屑78.5％,麸皮20％,石灰0.5％。共试验两批。第一批封口材料:a.无棉套盖;b.套环加棉塞。15×30cm的聚丙烯袋,高压灭菌。第二批封口材料:c.无棉套盖,d.棉线扎紧袋口。17×34cm的低压聚乙烯袋,常压灭菌。分别于1998年6月12、16日接毛木耳193,每组60袋,27～31℃培养。菌丝封面后隔7天测菌丝长速,各测2次,记录污染袋数。 3 试验结果 ①菌丝长速:接种后7～8天,菌丝封面,各组差异不显著。采用无棉盖体套盖的菌丝日均长速与用棉塞封口的差异不显著,但显著快于用棉线扎袋口 Plastic bags with plastic species, commonly used tampon or tie line sealing bag mouth, the former is susceptible to tamping when sterilization sterilization to increase the rate of pollution, while the latter due to poor ventilation caused by late hyphae slow, slender poor growth. We use Xiamen City, Xiamen Green Plastic Products Factory production of cotton cover plastic double-loop bacteria, and achieved good results. A cotton cover body structure points ring and cover two parts. The center of the lid has a diameter of 1.1cm hole, underlay a layer of cotton cover, under the cover of a sheet of 6 holes plastic sheet. Cover the collar when used, can be pressed. 2 test methods matrix formula for sawdust 78.5%, bran 20%, lime 0.5%. A total of two batches of experiments. The first batch of sealing material: a. No cotton cover; b. Collar plus tampons. 15 × 30cm polypropylene bags, autoclaved. The second batch of sealing materials: c. No cotton cover, d. 17 × 34cm low-pressure polyethylene bags, atmospheric sterilization. Respectively, on June 16, 1998 received Mao fungus 193, each bag 60 bags, 27 ~ 31 ℃ culture. Mycelium cover mycelial length of 7 days after the test, the measured 2 times, record the number of contaminated bags. 3 test results ① mycelium long-speed: 7 to 8 days after inoculation, mycelium cover, the difference was not significant in each group. The average daily speed of mycelium without cotton cover was not significantly different from that with tampon, but significantly faster than that with cotton thread