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本文阐述体外由植物血凝素（ＰＨＡ）激发的人外周血淋巴细胞化学发光的实验条件，并研究叠氮钠、松胞素Ｂ、秋水仙胺对淋巴细胞化学发光峰值的影响。结果表明，叠氮钠（１０，２０，４０ｍｍｏｌ／Ｌ。）能够通过抑制淋巴细胞膜ＮＡＤＰＨ氧化酶氧化磷酸化而减弱淋巴细胞化学发光（Ｐ＜０．０１）。松胞素Ｂ（１，５，１０μｇ／ｍｌ）和秋水仙胺（１，２，４μｇ／ｍｌ）分别破坏淋巴细胞的微管、微丝而抑制淋巴细胞化学发光（Ｐ＜０．０１）。由此可见，ＮＡＤＰＨ氧化酶氧化磷酸化，细胞骨架在淋巴细胞化学发光中起着重要的作用。
In this paper, the experimental conditions of chemiluminescence of human peripheral blood lymphocytes stimulated by phytohemagglutinin (PHA) in vitro and the effects of sodium azide, cytochalasin B and colchicine on the chemiluminescence peak of lymphocytes were studied. The results showed that sodium azide (10,20,40 mmol / L) attenuated lymphocyte chemiluminescence (P <0.01) by inhibiting the oxidative phosphorylation of NADPH oxidase on lymphocyte membrane. Cytokines (1, 5, 10μg / ml) and colchicine (1,2,4μg / ml) respectively destroys the microtubules and microfilaments of lymphocytes and inhibits lymphocyte chemiluminescence (P <0.01). Thus, NADPH oxidase oxidative phosphorylation, cytoskeleton in lymphocyte chemiluminescence plays an important role.