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目的 研究血管内皮细胞生长因子 (VEGF)与肝癌细胞转移的相互关系。方法 运用BodyenChamber膜侵袭培养系统培养VEGF肝癌细胞株HepG2 ,培养结束后 ,羊膜进行苏木精染色 ,计算穿过羊膜的下室肝癌细胞数以及停留在羊膜内的肝癌细胞数。结果 用VEGF 1ng/ml、5ng/ml、10ng/ml培养 5h ,下室浸润的肝癌细胞数分别为 (5 8± 1 0 )、(17 2± 2 4)、(10 30 9)× 10 4 /ml,分别高于对照组 (1 6± 0 4)× 10 4 /ml,P <0 0 5或 0 0 1。 5ng/mlVEGF培养 5h羊膜中浸润的肝癌细胞数为42个 ,与对照组 4个肝癌细胞比较 ,差异有非常显著意义 ,P <0 0 1。结论 VEGF可以诱导肝癌细胞转移 ,最佳浓度为 5ng/ml,最适培养时间为 2～ 5h ,VEGF诱导肝癌细胞转移机制与增强肿瘤细胞的迁移能力密切相关。
Objective To study the relationship between vascular endothelial growth factor (VEGF) and hepatoma cell metastasis. Methods VEGF hepatocellular carcinoma cell line HepG2 was cultured using the Bodyen Chamber membrane invasion culture system. After the culture was completed, the amniotic membrane was subjected to hematoxylin staining to calculate the number of hepatocellular carcinoma cells passing through the amniotic membrane and the number of hepatoma cells staying in the amniotic membrane. RESULTS: After incubation with VEGF 1ng/ml, 5ng/ml, and 10ng/ml for 5h, the number of hepatocellular carcinoma cells infiltrating into the lower chamber was (5 8 ± 1 0), (17 2 ± 2 4), (10 30 9) × 10 4 . /ml, respectively, higher than the control group (1 6 ± 0 4) × 10 4 /ml, P <0 0 5 or 0 0 1. The number of hepatoma cells infiltrating into the amniotic membrane at 5 ng/ml VEGF was 42. Compared with the 4 hepatoma cells in the control group, the difference was significant (P < 0.01). Conclusion VEGF can induce the metastasis of hepatocellular carcinoma cells, the optimal concentration is 5ng/ml, and the optimal culture time is 2～5h. The mechanism of VEGF-induced hepatoma cell metastasis is closely related to the enhancement of tumor cell migration.