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目的:研究端-端吻合术和耳大神经移植术修复面神经乳突段后再生纤维三维构筑特点与功能恢复的关系。方法:新西兰大白兔30只,随机分为端-端吻合组和耳大神经移植组,每组15只。对2组动物左侧耳分别实施面神经乳突段端-端吻合术或耳大神经移植术后30d、90d和120d检测肌电图,取双侧面神经乳突段及周围颞骨,脱钙,石蜡包埋,制作6μm连续切片后,利用Mimics 12.0软件重建显示再生神经的空间构筑。结果:2组动物比较再生纤维只有30d时差异有统计学意义,肌电图振幅30d和90d差异有统计学意义,三维重建结果显示随时间延长再生神经纤维髓鞘逐渐增厚、上下对接增多并聚集成簇。结论:不同修复术后面神经乳突段再生纤维三维构筑特点与功能逐渐恢复的趋势在时间上相应,但又不是完全一致。
OBJECTIVE: To study the relationship between the three-dimensional reconstruction of regenerated fibers and functional recovery after repairing the facial papillae with end-to-end anastomosis and large ear nerve transplantation. Methods: Thirty New Zealand white rabbits were randomly divided into end-to-end anastomosis group and auricular nerve transplantation group, 15 in each group. Electromyography was performed on the left ear of the two groups after 30 days, 90 days and 120 days after facial nerve mesentery end-to-end anastomosis or ear lumbar nerve transplantation respectively. Bilateral lateral mastoid process and the surrounding temporal bone, decalcification, paraffin After embedding and making 6μm serial sections, the space reconstruction of regenerative nerve was reconstructed by Mimics 12.0 software. Results: There was significant difference between the two groups of regenerated fibers in only 30 days. The EMG amplitudes of 30 days and 90 days were significantly different. Three-dimensional reconstruction showed that the myelin sheath of regenerated nerve fibers gradually thicken with time increasing, Clustered. CONCLUSIONS: The three-dimensional reconstruction features of facial nucleus pulposus axons and the gradual recovery of function in different repair phases correspond with each other in time, but they are not exactly the same.