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为了进一步研究精子抗原及其相应抗体在不育中的作用,本文用SDS-PAGE和免疫印迹法检查了一对长期不育夫妇之间的抗精子免疫应答。该夫妇双方均含高滴度抗精子抗体。丈夫精子膜蛋白提取液中分子量84KD蛋白含量显著增加,为正常男性的3～5倍。该成份在免疫印迹中可被夫妇双方血清特异性识别。提示不育男性精子抗原成份与生育力正常男子有量或质的差异,可能是产生抗精子抗体并造成不育的一个原因。
In order to further study the role of sperm antigens and their corresponding antibodies in infertility, we examined the anti-sperm immune response between a pair of long-term sterile couples by SDS-PAGE and Western blotting. Both couples contain high titers of anti-sperm antibodies. Husband sperm membrane protein extract 84KD molecular weight protein content increased significantly, compared with normal men 3 to 5 times. This composition can be specifically recognized by both spouses in the immunoblot. Suggest that male infertility male sperm antigen content and fertility normal men with quantitative or qualitative differences may be generated anti-sperm antibodies and cause infertility, a reason.