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为了明确桂枝茯苓胶囊治疗原发性痛经、盆腔炎和子宫肌瘤的主要药效成分,该研究基于分子烙印技术将桂枝茯苓胶囊中主要成分梯度敲除(分别敲除10%,30%,50%,70%,90%,100%)后,分别在离体子宫收缩、LPS诱导RAW264.7释放TNF-α、小鼠脾淋巴细胞(SPL)增殖及子宫肌瘤细胞增殖模型上考察桂枝茯苓胶囊活性的变化情况。结果表明,1梯度敲除芍药苷、丹皮酚和苦杏仁苷,桂枝茯苓胶囊抑制离体子宫收缩作用逐步减弱;2梯度敲除没食子酸、桂皮醛、五没食子酰葡萄糖和茯苓多糖,桂枝茯苓胶囊抑制TNF-α释放作用逐步减弱;3梯度敲除没食子酸、芍药苷、桂皮醛、槲皮素和茯苓多糖,桂枝茯苓胶囊促SPL细胞增殖作用逐渐减弱;4梯度敲除芍药苷、丹皮酚、五没食子酰葡萄糖和白芍苷,桂枝茯苓胶囊制剂体外抑制子宫肌瘤细胞的作用逐渐减弱。该研究提示,上述成分可能是桂枝茯苓胶囊发挥治疗原发性痛经、盆腔炎和子宫肌瘤作用的重要活性基础。
In order to clarify the main pharmacodynamic components of Guizhi Fuling Capsule for the treatment of primary dysmenorrhea, pelvic inflammatory disease and uterine fibroids, this study based on the molecular imprinting technique, the main components of Guizhi Fuling Capsule were knocked out (10%, 30% , 50%, 70%, 90% and 100% respectively), and then investigated in vitro models of uterine contractions, TNF-α release induced by LPS in RAW264.7, proliferation of mouse splenic lymphocytes (SPL) and proliferation of uterine fibroids Changes of activity of Guizhi Fuling Capsule. The results showed that 1 gradient of paeoniflorin, paeonol and amygdalin and Guizhi Fuling capsule inhibited the contractile effect of uterine contractions in vitro gradually; 2-step knock-out of gallic acid, cinnamaldehyde, five galloylglucose and tuckahoe polysaccharide, Zhiluoling Capsule can inhibit the release of TNF-α and gradually attenuate; 3 Gradient knock-out of gallic acid, paeoniflorin, cinnamic aldehyde, quercetin and tuckahoe polysaccharide, Guizhi Fuling capsule promote SPL cell proliferation gradually weakened; 4 gradient knockout paeoniflorin , Paeonol, five galloyl glucoside and white peonyone, Guizhi Fuling capsule inhibit the role of uterine fibroids in vitro gradually weakened. The study suggests that the above ingredients may be the main activity of Guizhi Fuling capsules play an important role in the treatment of primary dysmenorrhea, pelvic inflammatory disease and uterine fibroids.