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研究了低浓度(1、3、6、9μg/L)Cd2+长期(28 d)作用对泥蚶(Tegillarca granosa)内脏团和鳃两种组织的总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)及丙二醛(MDA)含量的影响。结果发现:与对照组相比,四个浓度组Cd2+对泥蚶鳃和内脏团两种组织的抗氧化酶体系均产生显著影响。除最高浓度组外,其余浓度组鳃和内脏团组织SOD活性前21 d均被诱导,第28 d时,所有组内脏团组织SOD活性被显著抑制,最高浓度组两种组织SOD活性第7d时被抑制,此后被诱导;除9μg/L浓度组外,其余浓度组鳃组织CAT活性与Cd2+浓度呈剂量效应关系,前14 d时被抑制,后14 d时被诱导;内脏团组织GSH-PX活性变化规律不明显;内脏团组织中产生的MDA含量显著高于鳃组织,所有组鳃组织中MDA含量均在第7 d时达到最高值,而内脏团组织中MDA含量仍在增加。研究发现,泥蚶鳃组织中CAT活性可以作为低浓度Cd2+污染的生物标志物;现今的国家渔业水质标准所规定的Cd2+浓度(≤0.005 mg/L)在泥蚶等双壳贝类上的适用性还有待探讨。
The effects of low concentrations of Cd2 + (1, 3, 6, 9μg / L) for 28 days on total superoxide dismutase (SOD) and hydrogen peroxide in visceral mass and gill of Tegillarca granosa (CAT), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) content. The results showed that compared with the control group, Cd2 + in four concentration groups had a significant effect on the antioxidant enzyme system in both gill and viscera groups. Except for the highest concentration group, the SOD activity in the gill and visceral mass tissues of the other concentration groups was induced before 21 d. At the 28th day, the SOD activity of the visceral mass tissues in all the groups was significantly inhibited. At the highest concentration, Was inhibited and then induced. Except for 9μg / L, the CAT activity in the gill tissue of the other concentration groups was dose-dependently correlated with the concentration of Cd2 +, and was inhibited at the 14th day and induced at 14th day. GSH-PX MDA content in visceral mass was significantly higher than that in gill. The content of MDA in gill of all the groups reached the highest value on the 7th day, but the content of MDA in visceral mass was still increasing. The results showed that the activity of CAT in the gill of Gracilaria euphorbiae could be used as a biomarker of Cd2 + pollution at low concentration. The current application of Cd2 + concentration (≤0.005 mg / L) in national fishery water quality standards for bivalve mollusc Sex yet to be explored.