骨肉瘤细胞是氟的主要作用靶细胞,该细胞功能较为活跃,可通过异常增殖来增加骨量及骨基质形成量,进而引发骨皮质增厚及骨质硬化等多种不良症状~([1])。MCM3在真核生物的DNA复制过程中发挥重要作用,且可反映骨细胞等多种细胞的增殖情况~([2])。对氟化物如何导致骨细胞活性增强、细胞增生、如何引发一系列病理变化进行研究具有重要的临床意义。为探讨氟对骨肉瘤细胞MCM3基因表达的影响,本研究 Osteosarcoma cells are the main target cells of fluoride. The cells are more active and can increase the amount of bone mass and bone matrix formation through abnormal proliferation, leading to various adverse symptoms such as cortical bone thickening and bone sclerosis. ]). MCM3 plays an important role in eukaryotic DNA replication and can reflect the proliferation of many kinds of cells, such as bone cells [2]. It is of great clinical significance to study how fluoride can lead to increased activity of bone cells, cell proliferation and how to initiate a series of pathological changes. To investigate the effect of fluoride on the gene expression of MCM3 in osteosarcoma cells, this study