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本文介绍了一种用体外培养的恶性疟原虫无性体作为活靶子细胞研究人疟特异抗体依赖性细胞毒性(ADCC)的微量测试方法。测试的对象有15名健康的对恶性疟有免疫力的未感染冈比亚成人(25～50岁);25名疟原虫血症为1～6%急性感染的冈比亚儿童(3个月～6岁);2名低度感染(<0.25%)恶性疟的冈比亚成人(20、21岁)。试验方法是从每个受检者取血6ml,其中1ml用于培养基和以后的抗体测定,其余5ml用肝素处理后,用F/H以400g作梯度高心15分钟分离单核细胞。95%的细胞为
This paper presents a micro-assay for studying human malarial specific antibody-dependent cellular cytotoxicity (ADCC) using live P. falciparum asexual cells as live target cells. Fifteen healthy, non-infected Gambian adults (25-50 years old) immunized against falciparum malaria were tested against 25 Gambian children (3 months to 6 years) with 25% of the malaria paralysis patients who were acutely infected with 1-6% ; 2 Gambian adults (20,21 years) with low-level infection (<0.25%) of P. falciparum. The test method is to take 6 ml of blood from each subject, 1 ml for culture medium and subsequent antibody assays, and the remaining 5 ml for treatment with heparin, followed by F / H separation with 400 g of graded centroblast for 15 minutes. 95% of the cells are