AIM:Mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today.Scientific research throughout the world has found evidence to support the fact that its foliar extracts have great potential against human microbial pathogens.This study highlights the isolation of foliar fungi from Rhizophora mucronata,Avicenna officialis and Avicenna marina.METHOD:It was isolated in Sabouroud’s Dextrose Agar and mass cultivation was done in Sabouroud’s Dex-trose broth.RESULTS:The ethyl acetate extract showed maximum antibacterial activity which inturn checked for different concentra-tion against bacterial pathogens and anticancer activity for Hep2 and MCF7 cell line in vitro.The DNA was isolated from the fungi and the ITS region of 5.8 s RNA was sequenced and assigned to new species as they are separated from the type strains phylogenetic neighbors by sequence similarities.CONCLUSION:This preliminary screening of fungal endophytes revealed their potential to yield potent bioactive compounds for drug discovery programmes.