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目的研究野菊花超临界二氧化碳萃取物(SCFE)对脂多糖(LPS)诱导RAW264.7细胞炎症反应的保护作用。方法体外培养RAW264.7细胞,LPS(100 ng·m L-1)诱导炎症模型,采用SCFE(75、150、300μg·ml~(-1))进行干预。MTT法测定细胞活性,Griess法测定细胞内一氧化氮(NO)水平,ELISA测定细胞内炎性介质PGE2及细胞因子(TNF-α、IL-1β、IL-6)的表达水平,RT-PCR法测定相关细胞因子和合成酶的m RNA表达。结果与空白对照组相比,LPS可显著增加RAW264.7细胞中NO、PGE_2以及TNF-α、IL-1β、IL-6的表达水平(P<0.05),而SCFE干预后上述指标均明显降低(P<0.05)。SCFE干预组中细胞内一氧化氮合成酶(i NOS)、环氧化酶-2(COX-2)及细胞因子TNF-α、IL-1β、IL-6的m RNA表达水平较LPS模型组显著下降(P<0.05)。结论 SCFE对LPS诱导的RAW264.7细胞炎症反应具有显著保护作用。
Objective To study the protective effect of supercritical carbon dioxide extract (SCFE) from Chrysanthemum morifolium on the inflammatory response induced by lipopolysaccharide (LPS) in RAW264.7 cells. Methods RAW264.7 cells were cultured in vitro. Inflammatory model was induced by LPS (100 ng · m L -1), and SCFE (75, 150, 300 μg · ml -1) was used for intervention. Cell viability was measured by MTT assay. Intracellular nitric oxide (NO) level was measured by Griess assay. PGE2 and cytokines (TNF-α, IL-1β and IL-6) Method for the determination of m RNA expression of related cytokines and synthase. Results Compared with the blank control group, LPS significantly increased NO, PGE 2, TNF-α, IL-1β and IL-6 expression in RAW264.7 cells (P <0.05) (P <0.05). Compared with LPS model group, the expression of iNOS, cyclooxygenase-2 (COX-2) and cytokines TNF-α, IL-1β and IL-6 in SCFE intervention group Significantly decreased (P <0.05). Conclusion SCFE has a significant protective effect on LPS-induced inflammatory response in RAW264.7 cells.