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目的探讨癌基因SALL4在苦参碱诱导人急性单核细胞白血病M5型THP-1细胞株凋亡过程中的表达及其意义。方法分别以1.0、1.5及2.0 g/L的苦参碱处理THP-1细胞,于24、48及72 h后,电镜观察细胞形态学改变;MTT法检测细胞增殖活性;流式细胞术检测细胞凋亡;RT-PCR检测细胞SALL4基因mRNA的表达。结果经1.0 g/L苦参碱作用24 h的THP-1细胞呈不同程度的凋亡形态改变;与对照组相比,各浓度苦参碱组均能显著抑制THP-1细胞增殖及促进细胞凋亡,且呈浓度和时间依赖性(P<0.05);各浓度苦参碱组SALL4基因mRNA的表达水平较对照组均显著下降,且呈浓度和时间依赖性(P<0.05)。结论 SALL4基因表达下降在苦参碱诱导白血病THP-1细胞凋亡过程中可能可能起重要作用。
Objective To investigate the expression of oncogene SALL4 in matrine-induced apoptosis in human acute monocytic leukemia M5 THP-1 cell line and its significance. Methods THP-1 cells were treated with matrine at 1.0, 1.5 and 2.0 g / L, respectively. The morphological changes of cells were observed by electron microscope at 24, 48 and 72 h. Cell proliferation was detected by MTT assay. Flow cytometry Apoptosis; SALL4 mRNA expression was detected by RT-PCR. Results THP-1 cells treated with 1.0 g / L matrine for 24 h had morphological changes of apoptosis. Compared with the control group, the concentrations of matrine in each group significantly inhibited the proliferation and promoted the proliferation of THP-1 cells (P <0.05). The expression of SALL4 mRNA in matrine group was significantly lower than that in control group (P <0.05) in a concentration-and time-dependent manner. Conclusion The decrease of SALL4 gene expression may play an important role in the apoptosis of leukemia THP-1 cells induced by matrine.