论文部分内容阅读
为研究丙二醇和4-羟基土烯醛同时修饰低密度脂蛋白后引起其结构和功能的变化，先用这两种醛类同时修饰低密度脂蛋白，再用琼脂糖凝胶电泳测定了其相对迁移率，然后制成特异性抗体，用放射性标记法测定了这种抗体对细胞降解醛类修饰低密度脂蛋白的影响。结果发现这种修饰脂蛋白的抗原性主要取决于丙二醇的修饰（约70％），与氧化型伙密度脂蛋白有一定程度的类同（约21％），但不同于乙酸化价密度脂蛋白。THP-1细胞降解125I-醛类修饰低密度脂蛋白的能力与125I-氧化型低密度脂蛋白相似，分别为243±53μg／g细胞蛋白和233±35μg／g细胞蛋白，但不如125I-未修饰低密度脂蛋白（441±16μg／g细胞蛋白）；乙酰化低密度脂蛋白能竞争性抑制125I-醛类修饰低密度脂蛋白的降解，而低密度脂蛋白则不能。在小鼠腹腔巨噬细胞，对125I-醛类修饰低密度脂蛋白的降解值约为125I-低密度脂蛋白的10倍，当加入不同浓度针对醛类修饰低密度脂蛋白的抗体后，发现细胞的降解值为原来的18％～223％，提示这种观醛修饰低密度脂蛋白可能主要通过细胞膜清道夫受体途径为细胞所摄取。
In order to study the structural and functional changes of low density lipoprotein induced by the simultaneous modification of propanediol and 4-hydroxytryprenal, the two aldehydes were used to modify low density lipoproteins simultaneously. The relative content of these two aldehydes was determined by agarose gel electrophoresis Mobility, and then made a specific antibody, radioactive labeled method was used to determine the effect of this antibody on cellular degradation of aldehyde-modified low-density lipoprotein. As a result, the antigenicity of this modified lipoprotein was found to be mainly dependent on the modification (about 70%) of propylene glycol and a certain degree of similarities (about 21%) to oxidized partial lipoprotein, but not the same . The ability of THP-1 cells to degrade 125I-aldehyde-modified low-density lipoproteins was similar to 125I-oxidized low-density lipoproteins, with 243 ± 53 μg / g cellular protein and 233 ± 35 μg / g cellular protein, respectively, Modified HDL (441 ± 16 μg / g cell protein); acetylated LDL competitively inhibited the degradation of 125I-aldehyde-modified LDL, whereas LDL did not. In mouse peritoneal macrophages, the degradation value of 125I-aldehyde-modified LDL is about 10 times that of 125I-LDL. After adding different concentrations of antibodies against aldehyde-modified LDL, it was found that Degradation of cells was 18% ~ 223% of the original, suggesting that the view of aldehyde-modified low-density lipoprotein may be mainly through the membrane scavenger receptor pathway for cellular uptake.