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建立了竞争抑制性酶联免疲吸附试验方法(CI—ELISA)以定量测定三硝基甲苯(TNT)血红蛋白加合物,该法的线性范围0.5 ng·ml~(-1)～1μg·ml~(-1),每个样品最小可测值为0.05 ng;批内变异系数8.0％左右,批间变异系数约为15.0％;正常大鼠血浆和血红蛋白溶液中外加2-氨基-4,6-二硝基甲笨(2A)或4-氨基-2,6-二硝基甲苯(4A)的回收率为84～101％,用此法首次检测TNT染毒大鼠中血红蛋白加合物,证实TNT血红蛋白加舍物水平与染毒剂量之间有明显的依赖关系,并可存留较长时间;反复染毒时,血红蛋白中2A,4A量有明显蓄积现象。
A competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) was developed for the quantitative determination of trinitrotoluene (TNT) hemoglobin adduct with a linear range of 0.5 ng · ml -1 -1 μg · ml ~ (-1), the minimum measurable value of each sample was 0.05 ng; the intra-assay coefficient of variation was 8.0% and the inter-assay coefficient of variation was about 15.0%; 2-Amino-4,6 The recovery of dinitrotoluene (2A) or 4-amino-2,6-dinitrotoluene (4A) was 84-101%. The hemoglobin adduct in TNT- Confirmed TNT hemoglobin Jiaxuewu levels and exposure dose between a significant dependence, and can be retained for a long time; repeated exposure to poisoning, hemoglobin 2A, 4A significant accumulation of the phenomenon.