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为确定缺铁非贫血大鼠是否存在脑组织生化异常，我们应用低铁饮食建立了铁缺乏非贫血期大鼠模型，用三电极等离子体原子发射直读光谱仪测定脑组织铁含量，酶组化法测定纹状体单胺氧化酶（ＭＡＯ）活性，高效液相色谱电化学检测器测定大脑皮层及海马中单胺类神经递质及其代谢产物的含量。结果显示，在铁缺乏非贫血期实验组大鼠脑组织铁含量及ＭＡＯ活性均明显降低，大脑皮层去甲肾上腺素及５－羟色胺（５－ＨＴ）含量显著高于对照组，海马中５－ＨＴ的代谢产物５—羟吲哚乙酸显著低于对照组。结果表明，在铁缺乏非贫血期大鼠脑组织已存在单胺类神经递质的代谢异常。本结果为铁缺乏所致行为智能发育异常提供了生化基础
To determine whether there is a biochemical abnormality of brain tissue in iron-deficient non-anemic rats, we established a model of iron-deficient non-anemia rats by using low-iron diet. The content of iron in brain tissue was measured by direct-reading three-electrode plasma atomic emission spectrometry. Method to determine the activity of monoamine oxidase (MAO) in the striatum. The contents of monoamine neurotransmitters and their metabolites in the cerebral cortex and hippocampus were determined by high performance liquid chromatography with electrochemical detector. The results showed that in the iron-deficient non-anemia period, the content of iron and the activity of MAO in the brain tissue of rats in the experimental group were significantly decreased. The contents of norepinephrine and 5-hydroxytryptamine (5-HT) in the cerebral cortex were significantly higher than those in the control group 5-hydroxyindoleacetic acid, a metabolite of HT, was significantly lower than that of the control group. The results show that there is metabolic abnormalities of monoamine neurotransmitters in brain tissue of rats with iron deficiency and non-anemia. This result provides a biochemical basis for abnormalities in the development of intelligence due to iron deficiency