论文部分内容阅读
目的探讨分析异质性万古霉素中介金黄色葡萄球菌(h-VISA)的分离检测方法,提高临床诊断率。方法选择2010年3月-2013年3月住院患者血流感染标本中金黄色葡萄球菌50株,采用简易菌群分析法筛选h-VISA,筛选阳性后检测耐甲氧西林金黄色葡萄球菌(MRSA)对万古霉素的MIC值。结果全部50株试验菌株中共有2株在4μg/ml万古霉素的M-H培养基培养48h后生长频率≥10-6,且在不含万古霉素的M-H平板上培养9代后MIC值仍能保持在中介耐药范围内,判定为h-VISA,检出率为4.0%。结论系统地监测h-VISA的流行情况,选择有效的检测方法,对指导临床正确使用万古霉素,有效控制h-VISA的传播有重要意义。
OBJECTIVE: To investigate the isolation and detection of heterogeneous vancomycin-mediated Staphylococcus aureus (h-VISA) and to improve the clinical diagnosis rate. Methods 50 strains of Staphylococcus aureus were selected from the blood samples of inpatients from March 2010 to March 2013. The h-VISA was screened by the simple colony analysis method, and then the methicillin-resistant Staphylococcus aureus (MRSA ) MIC against vancomycin. Results A total of 2 out of 50 strains of test strains were grown in 4μg / ml vancomycin medium for 48h and the growth frequency was ≥10-6. After cultured for 9 generations in vancomycin-free MH plates, Maintained within the scope of drug resistance, judged as h-VISA, the detection rate was 4.0%. Conclusion The systematic monitoring of the prevalence of h-VISA and the selection of effective detection methods are of great importance in guiding the proper use of vancomycin in clinical practice and effectively controlling the transmission of h-VISA.