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合成了四种双(N,N′-乙基,苯基)-2,3-萘-二(氧杂乙酰胺)(L=C30H30N2O4)稀土配合物。通过元素分析、红外光谱、差热-热重及摩尔电导值的分析,确定配合物的组成为[RE(pic)3L],RE=Eu(Ⅲ),Tb(Ⅲ),Ce(Ⅲ),Y(Ⅲ)。电化学实验表明:配体无电化学活性,配合物则呈现出一准可逆的氧化还原过程,在10～1000 mV.s-1扫速范围内配合物的氧化还原峰电流与扫描速度的平方根(ν1/2)呈良好的线性关系,表明其在玻碳电极(GCE)上的伏安行为受扩散控制。另外,通过循环伏安法、光谱法及粘度法研究了配合物与DNA的作用方式,结果表明:配合物均以插入方式与DNA作用,并且作用大小次序是Ce(pic)3L>Tb(pic)3L>Y(pic)3L>Eu(pic)3L。在pH=3.0适宜酸度条件下,建立了以Ce(pic)3L为探针定量测定DNA的分析方法。
Four rare earth complexes of bis (N, N’-ethyl, phenyl) -2,3-naphthalene-bis (oxaacetamide) (L = C30H30N2O4) were synthesized. The composition of the complex was determined as [RE (pic) 3L], RE = Eu (Ⅲ), Tb (Ⅲ) and Ce (Ⅲ) by elemental analysis, IR spectra, TG- Y (III). Electrochemical experiments show that the ligand has no electrochemical activity and the complex exhibits a quasi-reversible redox process. The square root of the redox peak current and scan rate of the complex in the range of 10 ~ 1000 mV.s-1 (ν1 / 2) showed a good linear relationship, indicating that its voltammetry at the glassy carbon electrode (GCE) diffusion control. In addition, the interaction between the complexes and DNA was studied by cyclic voltammetry, spectroscopy and viscometry. The results showed that all the complexes interacted with the DNA by insertion, and the order of action was Ce (pic) 3L> Tb (pic ) 3L> Y (pic) 3L> Eu (pic) 3L. Under pH = 3.0 suitable acidity conditions, a method for the quantitative determination of DNA using Ce (pic) 3L as a probe was established.