从生化角度,毛细胞的能量代谢是否由声信号所刺激的问题,成为被特别关注的焦点。作者取25只正常豚鼠,在基底转鼓阶和前庭阶打孔灌注人工外淋巴液、同时放入电极记录CM。将动物暴露于120dB S P L非生理性白噪声后,收集25～30μl灌注液,对葡萄糖、乳糖酶和丙酮酸盐进行生化分析,并测定耳蜗内氧分压。结果发现,CM记录到9/17的动物阈值有改变,并在7分钟内有暂时阈移。10只动物做代谢物评价,其中8只乳糖酶增加,3～4分钟时达高峰,继续声刺激时则减少。葡萄糖和丙酮酸盐无明显变化。耳蜗内氧分压亦无显著变化。作者认为灌注液的代谢物质,是来自包括内、外毛细胞在内的耳蜗细胞结构,它们的变化可以反映耳蜗的代谢。因为 From a biochemical point of view, whether the energy metabolism of hair cells is stimulated by acoustic signals has become the focus of particular attention. The author took 25 normal guinea pigs, perfused artificial perilymph at the basal drum and vestibular steps, and placed CM into the electrode. Animals were exposed to 120 dB S P L non-physiological white noise and 25 to 30 μl of perfusate were collected for biochemical analysis of glucose, lactase and pyruvate, and the oxygen partial pressure in the cochlea was measured. The results showed that CM recorded 9/17 animal threshold has changed, and within 7 minutes there is a temporary threshold shift. Ten animals were evaluated for metabolites, of which 8 lactase increased, peaked at 3 to 4 minutes, and continued to decrease upon acoustic stimulation. No significant changes in glucose and pyruvate. Oxygen pressure in the cochlea also did not change significantly. The author thinks that the metabolites of perfusate come from the cochlear cell structure including inner and outer hair cells, and their changes can reflect the cochlear metabolism. because