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利用离子束溅射镀膜技术将聚四氟乙烯(Teflon)纳米粒子沉积到石英基板,将基板置于50μg/ mL的瑞氏木霉疏水蛋白HFBII溶液中浸泡,通过原子力显微镜直接观察和水接触角测试仪测试基板表面水接触角的变化,研究了HFBII在Teflon纳米粒子表面的吸附行为.结果表明Teflon纳米粒子能够对HFBII产生吸附且对其生长有很强的异相成核作用,同时用超声波方法对Teflon纳米粒子上的HFBII进行脱附,并通过原子力显微镜图像进行了分析.
Teflon nanoparticles were deposited on a quartz substrate by ion beam sputter coating, the substrate was immersed in a 50 μg / mL solution of Trichoderma reesei hydrophobin HFBII, observed directly by atomic force microscopy and water contact angle TES was used to test the change of water contact angle on the surface of the substrate.The adsorption behavior of HFBII on the surface of Teflon nanoparticles was studied.The results showed that Teflon nanoparticles could adsorb HFBII and have a strong heterogeneous nucleation effect on the growth of Teflon nanoparticles, Methods HFBII on Teflon nanoparticles was desorbed and analyzed by atomic force microscopy.