论文部分内容阅读
本文结合1α,25双羟维生素D放射受体测定中所涉及的液闪测量问题,具体分析了生物医学样品液闪测量经常遇到,但又容易被忽视的一些问题。如果不正确处理这些问题,将导致实验结果分析的严重错误,如化学发光会使分步洗脱的放射层析谱产生假峰,不作DPM校正使样品失去可比性;不作本底扣除和本底淬灭校正,将使结果严重偏离真值;乳化测量的稳定性要求有一定的平衡时间。
In this paper, the problems of the measurement of liquid flashes involved in the determination of 1α, 25-dihydroxyvitamin D receptors are analyzed in detail, and some problems that are often encountered but often overlooked in the biomedical samples are also analyzed. Failure to properly address these issues can result in serious mistakes in assay results such as chemiluminescence that can lead to spurious peaks in fractionated eluent tomograms and loss of comparability without DPM calibration; no background subtraction and background Quenching the correction will result in a serious deviation from the true value; the stability of the emulsion measurement requires a certain amount of equilibration time.