1、向洗净的青霉素小瓶中加入大约5毫升清水。用打孔器从叶片中部打下小圆片,使叶片沉入瓶底。盖紧瓶盖。 2、用注射器针头插入瓶内,抽出叶片中的空气,直到叶片变为暗绿色呈浸渍状为止,此时叶肉细胞间隙内气体基本抽完。 3、将瓶内清水倒出来,换入约5毫升1％的NaHCO_3溶液,不再加盖。 1. Add approximately 5 ml of fresh water to the washed penicillin vial. Use a hole punch to make a small disc from the middle of the blade and sink the blade into the bottom of the bottle. Tighten the cap. 2. Insert the needle into the bottle with a syringe needle and withdraw the air from the leaves until the leaves turn dark green and impregnate. At this time, the gas in the mesenchymal cell gap is basically drawn. 3. Pour out the bottle of clear water and replace it with about 5 ml of a 1% solution of NaHCO3. Do not cap it.