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目的研究在冲突性应激条件下大鼠行为和内分泌的变化。方法56只成年雄性SD大鼠,随机分为8组,每组7只,利用Vogel冲突实验方法稍加修改,作为应激源,开场试验检测行为反应,测量体重,利用酶联免疫方法检测血清中皮质酮的含量。结果与对照组相比, 1周、2周、4周应激组大鼠表现为体重明显降低(216. 5±8. 71g, 194. 94±14. 82g, 259. 75±13. 83g):;在运动性方面, 1、2、4周应激组大鼠(67. 1±9. 2, 53. 66±6. 86, 59. 25±6. 46)有增加的趋势或者明显增加,在实验过程中,所有应激组大鼠皮质酮不同(1次应激组274. 41±25. 39ng.ml-1, 1周应激组240. 44±29. 51ng.ml-1, 2周应激组254. 21±37. 16ng.ml-1, 4周应激组161. 02±46ng.ml-1 )较对照组(89. 33±14. 04ng.ml-1 )明显升高。结论在应激大鼠表现的增多的运动,升高的皮质酮以及降低的体重,与焦虑障碍的某些特点相似,并且行为和内分泌的变化可以维持一段时间。冲突相关的应激可以作为研究焦虑及其他精神创伤应激比较理想的动物模型。
Objective To investigate the changes of behavior and endocrine in rats under the condition of conflict stress. Methods Fifty-six adult male Sprague-Dawley rats were randomly divided into 8 groups with 7 mice in each group. Vogels collision test was used to modify them as stressors. The opening test was used to measure the behavioral response, body weight was measured, and serum was detected by enzyme-linked immunosorbent assay In the corticosterone content. Results Compared with the control group, the rats in the stress group at 1 week, 2 weeks and 4 weeks showed significant weight loss (216. 5 ± 8. 71g, 194. 94 ± 14. 82g, 259. 75 ± 13. 83g) : In exercise, 1,2,4 and 4 weeks stress group rats (67.1 ± 9.2, 53.66 ± 6.86, 59.25 ± 6.46) showed an increasing trend or a significant increase , During the experiment, cortisone was different in all stress groups (274. 41 ± 25.39ng.ml-1 in the first stress group, 240.44 ± 29.51ng.ml-1 in the 1-week stress group, 2 weeks stress group 254. 21 ± 37 16ng.ml-1, 4 weeks stress group 161. 02 ± 46ng.ml-1) than the control group (89. 33 ± 14 04ng.ml-1) was significantly increased high. Conclusions The increased exercise, elevated corticosterone, and reduced body weight exhibited in stressed rats are similar to some of the characteristics of anxiety disorders and behavioral and endocrine changes can persist for some time. Conflict-related stress can be an ideal animal model for studying anxiety and other traumatic stress.