采用TNF体外细胞毒活性检测法发现,用BCG致敏ICR小鼠,再经腹腔注射不同剂量的口腔厌氧菌内毒素(LPS),可导致血清TNF迅速产生,其高峰位于注射后2h。腹腔注射1.0μg内毒素即可诱导机体产生TNF,并随内毒素剂量增加而呈剂量依赖曲线。至50μg内毒素时,TNF产生曲线趋于平坦,4种内毒素对TNF的诱导活性分别为4896～6580u/ml。 Using TNF in vitro cytotoxicity assay found that ICG sensitized with BCG mice, and then by intraperitoneal injection of different doses of oral anaerobic endotoxin (LPS), can lead to the rapid generation of serum TNF, peaked at 2h after injection. Intraperitoneal injection of 1.0μg of endotoxin can induce the body to produce TNF, and with a dose-dependent increase in endotoxic dose-dependent curves. When the concentration of lipopolysaccharide (LPS) was 50 μg, the TNF production curve tended to be flat. The inducing activities of four endotoxin to TNF were 4896 ~ 6580u / ml respectively.