【摘 要】
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Sec61β,a subunit of the Sec61 translocon complex,is not essential in yeast and commonly used as a marker of endoplasmic reticulum (ER).In higher eukaryotes,suc
【机 构】
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National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute
论文部分内容阅读
Sec61β,a subunit of the Sec61 translocon complex,is not essential in yeast and commonly used as a marker of endoplasmic reticulum (ER).In higher eukaryotes,such as Drosophila,deletion of Sec61β causes lethality,but its physiological role is unclear.Here,we show that Sec61β interacts directly with microtubules.Overexpression of Sec61β containing small epitope tags,but not a RFP tag,induces dramatic bundling of the ER and microtubule.A basic region in the cytosolic domain of Sec61β is critical for microtubule association.Depletion of Sec61β induces ER stress in both mammalian cells and Caenorhabditis elegans,and subsequent restoration of ER homeostasis correlates with the microtubule binding ability of Sec61β.Loss of Sec61β causes increased mobility of translocon complexes and reduced level of membrane-bound ribosomes.These results suggest that Sec61β may stabilize protein translocation by linking translocon complex to microtubule and provide insight into the physiological function of ER-microtubule interaction.
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