论文部分内容阅读
利用固相多肽合成中的 Boc和 Fmoc途径分别合成了胸腺素α1 (S- Tα1 )。后者的合成产率 (去保护基后粗肽量 /理论量 )为 80 % ,大于前者的 64.2 %。在 Fmoc方法合成中 ,用 Fmoc- Asn(Trt)上羟基树脂 (HMP树脂 )提高了合成产率。经 FPLC纯化后 ,Tα1 用 HPLC、毛细管电泳、等电聚焦电泳鉴定纯度均一 ;质谱和 N端序列分析证明其符合理论值。Tα1 活性测定 ,表明 S- Tα1 能提高淋巴细胞对丝裂原刺激的增殖反应 ,增强 NK细胞杀伤活性 ,具有增加淋巴细胞产生 IFN- γ的作用 ,我们的合成产物与国外对照品 Z- Tα1 呈现相仿的活性。此工作为进一步研究 Tα1 的免疫调节机理和探讨临床意义提供了基础
Thymosin α1 (S-Tα1) was synthesized by Boc and Fmoc in solid-phase peptide synthesis. The latter synthetic yield (deprotected crude peptide / theoretical amount) was 80%, 64.2% greater than the former. In the Fmoc method synthesis, the synthetic yield was increased with a hydroxyl resin (HMP resin) on Fmoc-Asn (Trt). After purified by FPLC, Tα1 was identified by HPLC, capillary electrophoresis and isoelectric focusing electrophoresis. The purity of Tα1 was confirmed by mass spectrometry and N-terminal sequence analysis. Tα1 activity assay showed that S-Tα1 can enhance the proliferation of lymphocytes response to mitogen stimulation and enhance the cytotoxic activity of NK cells, with increased lymphocyte production of IFN-γ, and our synthetic product with the foreign reference Z-Tα1 Similar activity. This work provided the basis for further study on the immune regulation mechanism of Tα1 and its clinical significance