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从我国北方春大豆区的黑龙江、吉林、辽宁、内蒙、河北承德以及北京采集典型症状的大豆叶片病样,经Top-Crop离体叶单斑分离以及DAS-ELISA抗血清免疫鉴定,共获得112个SMV阳性反应分离物。根据112个分离物在合丰25、文丰5号、诱变30、8101、铁丰25、Davis、Buffalo、早熟18、广吉、科丰1号、齐黄22共11个鉴别寄主上的症状反应,将我国北方春大豆区的SMV分成8个株系群。各株系群所占比例分析表明,北方春大豆区以SC-11株系群为主,其次为SC-8,各占病样总数的比例分别为51.7%和17.9%,SC-11和SC-8分别在黑龙江和辽宁分布最广,占当地株系群的比例分别为67.8%和75.0%。与以往株系划分系统的初步比较表明,SC-11与东北原1号株系群,SC-12和SC-16与东北原2号株系群,SC-4、SC-7、SC-8、SC-13和SC-17与东北原3号株系群在鉴别寄主上的反应相似。SC-16和SC-17为2个新发现的株系群。
Soybean leaf samples with typical symptoms collected in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei Chengde and Beijing in the spring soybean area of northern China were identified by Top-Crop isolated leaf spot and DAS-ELISA antiserum to obtain 112 SMV positive reaction isolates. Based on 112 isolates identified on 11 hereditary hosts at Hefeng 25, Wenfeng 5, mutagen 30,8101, Tiefeng 25, Davis, Buffalo, precocious 18, Kwongji, Kefeng 1, Qihuang 22 Symptom response, the northern part of China’s spring soybean area SMV is divided into 8 groups. The analysis of proportion of each lineage showed that the SC-11 lineage was the main group in northern spring soybean area, followed by SC-8, accounting for 51.7% and 17.9% of the total number of pathological lines respectively. The SC-11 and SC -8 were the most widely distributed in Heilongjiang and Liaoning, respectively, accounting for 67.8% and 75.0% of the local lineages respectively. The preliminary comparison with the pasting system indicated that the SC-11 and Northeast No. 1 strain group, SC-12 and SC-16 strains and Northeast Tohoku 2 strain group, SC-4, SC-7 and SC-8 , SC-13 and SC-17 and northeastern original 3 strains in the host response to identify similar. SC-16 and SC-17 are two newly discovered strains.