为了提高肾综合征出血热 (HFRS)纯化疫苗中糖膜蛋白 (GP)的纯度 ,采用调节pH值使乳鼠脑组织蛋白沉淀的方法 ,对蔗糖密度梯度区带离心脱糖后的GP样品进一步纯化。试验结果表明 ,此方法简便易行 ,能大幅度降低样品中的杂蛋白含量、提高GP纯度。以该法纯化前、后的样品免疫家兔 ,产生的血清中和抗体水平基本一致。用此方法 ,可省去部分昂贵的纯化设备 ,降低生产成本 ,为HFRS纯化疫苗的批量生产提供又一简单可行的途径。 In order to improve the purity of glyco-membrane protein (GP) in HFRS purified vaccine, a method of adjusting the pH value to precipitate protein in brain tissue of neonatal rats was used to further de-glycosylated GP samples in the sucrose density gradient zone purification. The test results show that this method is simple and easy, can significantly reduce the impurity content of the sample, improve the purity of GP. Immunization of rabbits with the samples before and after purification by this method yielded essentially the same level of serum neutralizing antibodies. With this method, part of expensive purification equipment can be omitted to reduce the production cost and provide another simple and feasible method for mass production of HFRS purified vaccine.