目的:视网膜缺血是临床常见病之一,目前有关视网膜不完全性缺血情况下细胞凋亡和凋亡相关基因表达的尚未见研究报告.方法:通过结扎双侧颈总动脉造成大鼠视网膜不完全性缺血1、3、5、14、30、60天,用TUNEL方法标记视网膜凋亡细胞,并用体视学方法进行定量分析,用免疫组化方法观察bcl-2的表达情况.结果:1.缺血1天节细胞层和内核层即见凋亡细胞,以内核层为多;3天在外核层也出现.7天与3天无明显差别.14天凋亡细胞增多,达高峰,术后30及60天凋亡细胞的数密度与14天无明显差异.2.缺血1天Muller’s细胞内侧部bc-2表达增强,包括终足及位于节细胞层的突起.内界膜处着色明显,节细胞层出现“斑、点、线”状和一些细胞样阳性着色结构.3天和7天,着色区域扩大至Muller’s细胞外侧部,在外界膜呈现一条着色线,外核层出现垂直走向的着色纤维,外网层出现着色网.14天bcl-2阳性反应局限于Muller’s细胞内侧部,节细胞层内细胞样着色团块增多,Muller’s细胞外侧部着色不明显.缺血60天,bcl-2表达明显 OBJECTIVE: Retinal ischemia is one of the most common clinical diseases.Now the research reports about apoptosis and apoptosis related gene expression in the case of incomplete retinal ischemia.Methods: The rat retinas were ligated by ligation of bilateral common carotid arteries 1, 3, 5, 14, 30 and 60 days after ischemia, retinal apoptotic cells were labeled with TUNEL method, and the expression of bcl-2 was observed by stereological methods and immunohistochemistry .Results : 1. The ischemic 1 day cell layer and the inner nuclear layer that apoptotic cells to the inner nuclear layer is more; 3 days in the outer nuclear layer also appeared 7 days and 3 days no significant difference in 14 days apoptotic cells increased up to The number density of apoptotic cells at 30 and 60 days after operation was not significantly different from that at day 14. 2. The expression of bc-2 in the medial part of Muller’s cells increased at 1 day after ischemia, Membrane staining was obvious, the section of the cell layer “spot, spot, line” shape and some cell-like positive staining structure.3 days and 7 days, the coloring area expanded to Muller’s cell lateral part, in the outer membrane showed a colored line, the outer core Stratified vertical layer of pigmented fibers, the outer network appears colored mesh .14 days bcl-2 Yang The reaction was confined to the medial part of Muller’s cells, the number of cell-like coloration increased in the cell layer, and the outer part of Muller’s cells was not stained.The expression of bcl-2 was significant at 60 days after ischemia