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目的:探讨DNA甲基转移酶抑制剂5-杂氮-2′-脱氧胞苷(5-aza-CdR)对人食管癌细胞株Eca109增殖作用的影响。方法:应用不同浓度(10-7、10-6、10-5、10-4mol/L)的特异性DNA甲基转移酶抑制剂5-aza-CdR,于不同作用时间(24～168h)处理人食管鳞癌细胞株Eca109,采用四甲基偶氮唑蓝(MTT)法测定5-aza-CdR作用下Eca109细胞株的生存率,倒置显微镜观察细胞的形态学变化。结果:不同药物浓度组在同一作用时间下,细胞株生存率差异有统计学意义(P<0.05);同一药物浓度不同作用时间组之间(24～96h)的细胞生存率差异也有统计学意义(P<0.05),且96h时对人食管鳞癌细胞株Eca109生长抑制作用最明显。形态学观察显示:癌细胞体积缩小,核固缩,染色质凝聚成块。结论:5-aza-CdR有抑制人食管癌细胞株Eca109生长的作用,抑制作用呈时间依赖性及浓度依赖性;并且干预后癌细胞呈现典型的凋亡细胞形态改变。
AIM: To investigate the effect of 5-aza-2’-deoxycytidine (DNA-methyltransferase 5-aza-CdR) on the proliferation of human esophageal cancer cell line Eca109. Methods: 5-aza-CdR, a specific DNA methyltransferase inhibitor with different concentrations (10-7, 10-6, 10-5 and 10-4 mol / L) Human esophageal squamous cell carcinoma cell line Eca109 was used to determine the survival rate of Eca109 cells treated with 5-aza-CdR by MTT method. The morphological changes of cells were observed with inverted microscope. Results: There was significant difference in cell survival rate between different drug concentration groups at the same action time (P <0.05). There was also significant difference in cell survival rate between different time points (24 ~ 96h) (P <0.05), and the inhibitory effect on the growth of human esophageal squamous carcinoma cell line Eca109 was the most obvious at 96h. Morphological observation showed that the cancer cells reduced in size, nuclear pyknosis and chromatin condensed into lumps. CONCLUSION: 5-aza-CdR can inhibit the growth of human esophageal cancer cell line Eca109 in a time-dependent manner and in a concentration-dependent manner. The morphological changes of typical apoptotic cells were observed after the intervention.