论文部分内容阅读
目的:建立用高效液相色谱同时测定赤砂糖中的过氧化苯甲酰和苯甲酸的方法。方法:采用Shim-pack C18色谱柱(5μm,4.6 mm×250 mm);以0.1%磷酸-甲醇(25∶75)为流动相;流速1.0 ml/min;检测波长为235 nm;柱温:室温。结果:过氧化苯甲酰和苯甲酸的最低检出限分别为0.11 mg/kg和0.15 mg/kg,平均回收率分别为100.71%和98.55%,RSD分别为0.78%和1.22%。结论:该方法具有准确可靠、操作简便的特点,可有效用于赤砂糖中的过氧化苯甲酰和苯甲酸的检测。
Objective: To establish a method for simultaneous determination of benzoyl peroxide and benzoic acid in red sugar by high performance liquid chromatography. Methods: A Shim-pack C18 column (5 μm, 4.6 mm × 250 mm) was used. The mobile phase consisted of 0.1% phosphoric acid and methanol (25:75) at a flow rate of 1.0 ml / min. The detection wavelength was set at 235 nm. . Results: The detection limits of benzoyl peroxide and benzoic acid were 0.11 mg / kg and 0.15 mg / kg, respectively. The average recoveries were 100.71% and 98.55%, respectively, with RSDs of 0.78% and 1.22%, respectively. Conclusion: The method is accurate and reliable, easy to operate and can be effectively used for the determination of benzoyl peroxide and benzoic acid in red sugar.