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〔目的〕调查湖北地区出口猪以及不同人群中戊型肝炎病毒的流行情况,为制定预防措施提供依据。〔方法〕从湖北地区8个出口猪场中共采集3个月龄以下猪的血清203份,3个月以上猪的血清637份。从不同人群(包括猪场和屠宰厂从业人员、农村居民以及献血员中共采集1585份血清样品。用酶联免疫方法分别检测戊型肝炎病毒抗体以及抗原,对部分样品采用实时荧光聚合酶链反应检测戊型肝炎病毒核酸。〔结果〕3个月龄以下和3个月龄以上猪血清中戊型肝炎病毒抗体的阳性率分别为56.65%和87.13%,2者间差异具有显著性(t=7.79,p<0.01);戊型肝炎病毒抗原的阳性率分别为9.35%和10.36%,2者间差异无显著性。在328份猪血清中,抗原和核酸的符合率为78.80%。猪场和屠宰厂从业人员、农村居民以及献血员中戊型肝炎病毒IgG抗体的阳性率分别为55.72%(263/472)、31.63%(99/313)、27.00%(81/300)和33.40%(167/500)。猪场从业人员戊型肝炎病毒IgG抗体阳性率与其他3类人群的相比,具有非常显著性差异(t=8.88,p<0.01)。〔结论〕猪群中戊型肝炎感染率较高,与猪密切接触人群的感染率高于其他人群。因此,应加强出口猪场的管理,减少戊型肝炎病毒在人畜之间的传播。
[Objective] To investigate the prevalence of hepatitis E virus in export pigs and different populations in Hubei Province, and provide the basis for preventive measures. [Methods] Totally 203 serum samples from 3 pigs aged less than 3 months and 637 pigs from 3 pigs were collected from 8 export farms in Hubei Province. A total of 1585 serum samples were collected from different populations (including employees in pig farms and slaughterhouses, rural residents and blood donors). Anti-HEV antibodies and antigens were detected by enzyme linked immunosorbent assay (ELISA), and real-time fluorescence polymerase chain reaction The results showed that the positive rate of hepatitis E virus antibody in pig serum of 3 months old and 3 months old were 56.65% and 87.13%, respectively, the difference was significant (t = 7.79, p <0.01). The positive rates of hepatitis E virus antigen were 9.35% and 10.36%, respectively. There was no significant difference between the two groups. The coincidence rate of the antigen and nucleic acid was 78.80% The positive rates of hepatitis E virus IgG antibodies among slaughterhouse workers, rural residents and blood donors were 55.72% (263/472), 31.63% (99/313), 27.00% (81/300) and 33.40% (T = 8.88, p <0.01). [Conclusion] The prevalence of hepatitis E virus IgG in pig farms was significantly higher than that in the other three groups (t = 8.88, p <0.01) Infection rate is higher, the close contact with pigs infection rate is higher than other groups. This should strengthen the export of farm management, reduce the spread of hepatitis E virus between humans and animals.