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为了研究植物挥发性化合物α-萜品醇的杀虫活性及作用机理,采用熏蒸法测定α-萜品醇对黄粉虫幼虫的急性毒性,并测定处理不同时间点对其体内3种酶活性的影响。试验结果表明,α-萜品醇对黄粉虫幼虫12、24、36 h和48 h的LC50分别为121.597、105.798、73.718μg/L和49.332μg/L。用亚致死浓度LC20、致死中浓度LC50的α-萜品醇对黄粉虫体内羧酸酯酶(Car E)、酸式磷酸酯酶(ACP)、碱式磷酸酯酶(ALP)、谷胱甘肽-s-转移酶(GSTs)12、24、36、48 h的影响存在差异性,但均表现出抑制功能。综合时间、剂量效应及酶受到抑制的程度来看,3种酶对α-萜品醇胁迫的敏感性从大到小依次为GSTs>ALP>ACP>Car E。上述结果表明,α-萜品醇对黄粉虫有较高的生物活性,能有效干扰其体内酶系统,扰乱其正常生理代谢,表现出较高的毒杀效果。
In order to study the insecticidal activity and mechanism of α-terpineol, a volatile compound of plant, the acute toxicity of α-terpineol to Tenebrio molitor larvae was determined by fumigation method. The activities of three enzymes in vivo were measured at different time points influences. The results showed that the LC50 of α-terpineol at 12, 24, 36 h and 48 h were 121.597, 105.798, 73.718μg / L and 49.332μg / L, respectively. The inhibitory effects of α-terpineol with LC50 of lethal concentration and LC50 on the CarE, ACP, ALP, The effects of peptide-S-transferase (GSTs) at 12, 24, 36 and 48 h were different, but all showed inhibitory function. The results showed that the sensitivity of the three enzymes to α-terpineol stress was GSTs> ALP> ACP> CarE in descending order of time, dose effect and enzyme inhibition. The above results indicate that α-terpineol has higher biological activity on Tenebrio molitor, can effectively interfere with its in vivo enzyme system, disrupt its normal physiological metabolism, showing higher toxicity.