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目的运用ROC曲线确定磁分离酶联免疫法(MPAIA)在血吸虫病临床免疫诊断中的最佳临界值。方法选择湖北省荆州市部分居民,用病原学和MPAIA方法同时对该人群进行检查。同时采用MPAIA方法对确诊的钩虫病、囊尾蚴病、旋毛虫病及乙肝患者的血清进行检测。用ROC曲线确定MPAIA在血吸虫病诊断中的最佳临界值。结果共检测549人,有疫水接触史的224人中,病原学检测阳性79人,MPAIA检测阳性92人;无疫水接触史的185人中,病原学检测均为阴性,MPAIA检测阳性2人。MPAIA法检测钩虫病、囊尾蚴病、旋毛虫病与乙肝患者血清均为阴性。ROC曲线确定血吸虫病诊断的最佳临界值为0.403(550nm),曲线下面积为0.923。结论 MPAIA在血吸虫病诊断中的最佳临界值为0.403(550nm),但应结合专业知识及实际情况来确定临床最佳临界值。
Objective To determine the best cutoff value of magnetic resonance immunoassay (MPAIA) in clinical immunodiagnosis of schistosomiasis by ROC curve. Methods Selected residents of Jingzhou City, Hubei Province, using etiology and MPAIA method to simultaneously examine the population. At the same time, MPAIA was used to detect the serums of confirmed hookworms, cysticercosis, trichinosis and hepatitis B patients. ROC curve was used to determine the best cutoff value of MPAIA in the diagnosis of schistosomiasis. Results A total of 549 persons were detected, of which 224 were infected with water, 79 were positive for pathogen detection and 92 were positive for MPAIA. Of the 185 persons without history of exposure to water, the etiological test was negative and the MPAIA test was positive people. MPAIA test hookworm disease, cysticercosis, trichinosis and hepatitis B patients were negative serum. The best cutoff value of ROC curve for diagnosis of schistosomiasis was 0.403 (550nm), and the area under the curve was 0.923. Conclusion The best critical value of MPAIA in the diagnosis of schistosomiasis is 0.403 (550nm), but the best clinical critical value should be determined according to the professional knowledge and the actual situation.