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目的 :研究可溶性syndecan 1(CD138)分子对人多发性骨髓瘤 (MM)细胞生长行为的影响及其机制。方法 :用亲和层析法分离可溶性syndecan 1;借助3H TdR掺入、间接免疫荧光、ANNEXIN V及PI标记分析MM细胞增殖、凋亡及周期变化。结果 :①从XG 2细胞培养上清中分离纯化得到分子量为 6 0kD左右的可溶性syndecan 1分子 ;②可溶性syndecan 1分子在体外能抑制XG 1增殖 ,阻滞细胞增殖停留在G2 /M期 ,并介导其凋亡 ;FGF、5 %小牛血清、肝素酶III可逆转一定浓度可溶性syn decan 1的作用 ,HGF、VEGF和IGF 1可部分逆转可溶性syndecan 1分子对细胞的生长抑制作用 ;③IL 6及激发型抗IL 6R130(gp130 )单抗对可溶性syndecan 1作用无影响。结论 :可溶性syndecan 1分子通过不同于IL 6及其受体的信号传导途径 ,参与了人多发性骨髓瘤细胞的生长调控
Objective: To study the effect of soluble syndecan 1 (CD138) on the growth behavior of human multiple myeloma (MM) cells and its mechanism. METHODS: Soluble syndecan 1 was separated by affinity chromatography; 3H TdR incorporation, indirect immunofluorescence, ANNEXIN V and PI markers were used to analyze the proliferation, apoptosis and cycle of MM cells. RESULTS: 1The soluble syndecan 1 with a molecular weight of about 60 kD was isolated and purified from the culture supernatant of XG 2 cells; 2 soluble syndecan 1 could inhibit the proliferation of XG 1 in vitro, and arrested the proliferation of cells in the G2 /M phase. Mediate its apoptosis; FGF, 5% calf serum, heparinase III can reverse the effect of soluble syn decan 1, and HGF, VEGF and IGF 1 can partially reverse the growth inhibitory effect of soluble syndecan 1 on cells; 3IL 6 and activated anti-IL 6R130 (gp130) monoclonal antibody had no effect on soluble syndecan 1. CONCLUSION : Soluble syndecan 1 is involved in the growth regulation of human multiple myeloma cells through a signal transduction pathway that is different from IL 6 and its receptors.