目的：建立优化ＨＬＡＡ位点ＰＣＲＳＳＰ分型方法。方法：采用普通扩增仪和Ｅｐｐｅｎｄｏｒｆ管对细胞系ＤＮＡ和３７个健康正常人进行基因分型，血清学检测采用标准淋巴细胞毒试验方法。结果：发现引物浓度和浓度比、ＤＮＡ的纯度及酶的选用，是影响ＨＬＡＡ位点ＰＣＲＳＳＰ分型准确性的重要因素。该方法对３７个标本的基因分型结果与血清学结果相符合。结论：ＰＣＲＳＳＰ方法具有简便准确的优点，可以作为ＨＬＡＡ位点分型的一种新途径并推广应用 OBJECTIVE: To establish a PCRSSP typing method to optimize HLAA locus. Methods: Genotyping of cell line DNA and 37 healthy normal subjects was performed by using general amplification instrument and Eppendorf tube. Serological tests were performed by standard lymphocytotoxicity test. Results: It was found that the primer concentration and concentration ratio, DNA purity and enzyme selection, is an important factor affecting the PCR  A site PCR SPC typing accuracy. The method of 37 specimens of genotyping results and serological results. Conclusion: PCR-SP method has the advantages of simple and accurate, as a HLA A site typing a new approach and promote the use of